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Abbreviation : DEPC
Long Form : diethyl pyrocarbonate
No. Year Title Co-occurring Abbreviation
2020 Copper(II) partially protects three histidine residues and the N-terminus of amyloid-beta peptide from diethyl pyrocarbonate (DEPC) modification. MALDI-TOF MS
2020 Covalent Labeling/Mass Spectrometry of Monoclonal Antibodies with Diethylpyrocarbonate: Reaction Kinetics for Ensuring Protein Structural Integrity. mAbs
2020 Extracellular lysine 38 plays a crucial role in pH-dependent transport via human monocarboxylate transporter 1. DIDS, hMCTs, PGO
2020 Higher-Order Structure Influences the Kinetics of Diethylpyrocarbonate Covalent Labeling of Proteins. CL, MS
2020 Highly degraded RNA can still provide molecular information: An in vitro approach. ---
2019 Atroxlysin-III, A Metalloproteinase from the Venom of the Peruvian Pit Viper Snake Bothrops atrox (Jergon) Induces Glycoprotein VI Shedding and Impairs Platelet Function. Atr-III, GPVI, vWF
2019 Covalent labeling and mass spectrometry reveal subtle higher order structural changes for antibody therapeutics. CL, MS
2019 Covalent Labeling with Diethylpyrocarbonate: Sensitive to the Residue Microenvironment, Providing Improved Analysis of Protein Higher Order Structure by Mass Spectrometry. ---
2019 DEPC modification of the CuA protein from Thermus thermophilus. ---
10  2019 Synergistic Structural Information from Covalent Labeling and Hydrogen-Deuterium Exchange Mass Spectrometry for Protein-Ligand Interactions. CL, HDX, MS
11  2018 An improved method for studying whole sections of late developing wheat grain. ---
12  2018 Interaction of Cu+2 with alpha-Crystallin: A Biophysical and Mass Spectrometric Study. DLS
13  2017 A protease-resistant alpha-galactosidase from Pleurotus djamor with broad pH stability and good hydrolytic activity toward raffinose family oligosaccharides. NBS, PCMB, PDGI
14  2017 Alkyl-substituted phenylamino derivatives of 7-nitrobenz-2-oxa-1,3-diazole as uncouplers of oxidative phosphorylation and antibacterial agents: involvement of membrane proteins in the uncoupling action. AGC, NBD, NBD-Cn
15  2017 Mercury(II) binds to both of chymotrypsin's histidines, causing inhibition followed by irreversible denaturation/aggregation. ---
16  2017 Production and Characterization of Organic Solvent-Tolerant Cellulase from Bacillus amyloliquefaciens AK9 Isolated from Hot Spring. CMC, EDTA, SDS-PAGE
17  2017 Reactive sites and course of reduction in the Rieske protein. ---
18  2016 How the imidazole ring modulates amyloid formation of islet amyloid polypeptide: A chemical modification study. hIAPP, REMD
19  2015 Chemical modification interference. ---
20  2015 Diethylpyrocarbonate modification reveals HisB5 as an important modulator of insulin amyloid formation. ---
21  2015 Huntingtin N-Terminal Monomeric and Multimeric Structures Destabilized by Covalent Modification of Heteroatomic Residues. IMS-MS, MDS
22  2015 Investigating Therapeutic Protein Structure with Diethylpyrocarbonate Labeling and Mass Spectrometry. ---
23  2015 Methionyl-Methionine Promotes alpha-s1 Casein Synthesis in Bovine Mammary Gland Explants by Enhancing Intracellular Substrate Availability and Activating JAK2-STAT5 and mTOR-Mediated Signaling Pathways. AAs, alphas1-CN, APN, JAK2, Met-Met, mTOR, STAT5
24  2014 Application of monoclonal antibodies generated against Panton-Valentine Leukocidin (PVL-S) toxin for specific identification of community acquired methicillin resistance Staphylococcus aureus. CA-MRSA, PVL, Spa
25  2014 Involvement of Histidine Residue His382 in pH Regulation of MCT4 Activity. MCT4
26  2014 Label scrambling during CID of covalently labeled peptide ions. CID
27  2014 The reduction rates of DEPC-modified mutant Thermus thermophilus Rieske proteins differ when there is a negative charge proximal to the cluster. ---
28  2013 Characterization of a halostable endoglucanase with organic solvent-tolerant property from Haloarcula sp. G10. PMSF
29  2013 Importance of His192 in the human thyroid hormone transporter MCT8 for substrate recognition. ECL, MCT8, TH, TMD
30  2012 Detection of Mycobacterium tuberculosis directly from sputum specimens & phenotypic drug resistance pattern of M. tuberculosis isolates from suspected tuberculosis patients in Chennai. RT-PCR
31  2012 Diethylpyrocarbonate labeling for the structural analysis of proteins: label scrambling in solution and how to avoid it. ---
32  2012 Heterologous production and characterisation of two distinct dihaem-containing membrane integral cytochrome b(561) enzymes from Arabidopsis thaliana in Pichia pastoris and Escherichia coli cells. cyt
33  2012 Identification of histidine residues involved in Zn(2+) binding to alphaA- and alphaB-crystallin by chemical modification and MALDI TOF mass spectrometry. ---
34  2012 Increased protein structural resolution from diethylpyrocarbonate-based covalent labeling and mass spectrometric detection. ---
35  2012 Mass spectral studies reveal the structure of Abeta1-16-Cu2+ complex resembling ATCUN motif. His
36  2012 Nucleosome exclusion from the interspecies-conserved central AT-rich region of the Ars insulator. ---
37  2012 Purification and characterization of novel organic-solvent-tolerant beta-amylase and serine protease from a newly isolated Salimicrobium halophilum strain LY20. ow, PAO
38  2011 Cu2+ triggers reversible aggregation of a disordered His-rich dehydrin MpDhn12 from Musa paradisiaca. CD, IMAC
39  2011 Extracellular production of beta-amylase by a halophilic isolate, Halobacillus sp. LY9. EDTA, PAO, SDS
40  2011 Involvement of NADPH-dependent and cAMP-PKA sensitive H+ channels in the chorda tympani nerve responses to strong acids. CT, KO, PKA, PMA, WT
41  2011 Rapid detection of viable M. tuberculosis directly from sputum by reverse transcriptase PCR targeting 85B gene. M. tuberculosis, RT-PCR
42  2011 Study of biochemical pathway and enzyme involved in metsulfuron-methyl degradation by Ancylobacter sp. XJ-412-1 isolated from soil. BSM, MSM, PMSF, TSM
43  2010 Chemical modification of the Rieske protein from Thermus thermophilus using diethyl pyrocarbonate modifies ligating histidine 154 and reduces the [2FE-2S] cluster. ---
44  2010 Detection and quantification of Panton-Valentine leukocidin in Staphylococcus aureus cultures by ELISA and Western blotting: diethylpyrocarbonate inhibits binding of protein A to IgG. ELISA, PVL
45  2009 Allosteric modulation of 5-HT(1A) receptors by zinc: Binding studies. NEM
46  2009 Solution structure of physiological Cu(His)2: novel considerations into imidazole coordination. ---
47  2009 The use of diethyl pyrocarbonate and potassium permanganate as probes for strand separation and structural distortions in DNA. ---
48  2009 Viral inactivation based on inhibition of membrane fusion: understanding the role of histidine protonation to develop new viral vaccines. VSV
49  2008 Characterization of heme-coordinating histidyl residues of an engineered six-coordinated myoglobin mutant based on the reactivity with diethylpyrocarbonate, mass spectrometry, and electron paramagnetic resonance spectroscopy. ---
50  2008 Extraction of RNA from dictyostelium. RNAi, RT-PCR
51  2008 Protein surface mapping using diethylpyrocarbonate with mass spectrometric detection. ---
52  2008 Role of histidine residues in EcoP15I DNA methyltransferase activity as probed by chemical modification and site-directed mutagenesis. ---
53  2007 A base-catalyzed mechanism for dark state recovery in the Avena sativa phototropin-1 LOV2 domain. AsLOV2, FMN, LOV
54  2007 A role for His155 in binding of human prion peptide144-167 to immobilised prion protein. rHuPrP, SPR
55  2007 Identification of essential histidine and cysteine residues of the H+/organic cation antiporter multidrug and toxin extrusion (MATE). MATE, MATE1, PCMBS, TEA
56  2007 Inactivation of vesicular stomatitis virus through inhibition of membrane fusion by chemical modification of the viral glycoprotein. His, VSV
57  2007 New chemical method of viral inactivation for vaccine development based on membrane fusion inhibition. VSV
58  2007 Preparing glass slides and coverslips for in situ hybridization. TESPA
59  2007 Transmembrane domain histidines contribute to regulation of AE2-mediated anion exchange by pH. His, TMD
60  2006 Broad-spectrum antioxidant peptides derived from His residue-containing sequences present in human paraoxonase 1. AAPH, Cys, HOCl, LDL, PON1, Tyr
61  2006 Diethyl pyrocarbonate, a histidine-modifying agent, directly stimulates activity of ATP-sensitive potassium channels in pituitary GH(3) cells. ATP, ATP
62  2006 Evidence for allosteric regulation of pH-sensitive System A (SNAT2) and System N (SNAT5) amino acid transporter activity involving a conserved histidine residue. ---
63  2006 Functional contribution of a conserved, mobile loop histidine of phosphoribulokinase. PRK
64  2006 Identification of oxidized methionine sites in erythrocyte membrane protein by liquid chromatography/electrospray ionization mass spectrometry peptide mapping. DNDS
65  2006 Imidazole binding reagent diethyl pyrocarbonate (DEPC) inhibits avian intrapulmonary chemoreceptor discharge in vivo. CA, IPC
66  2006 Influence of a proton gradient on the transport kinetics of the H+/amino acid cotransporter PAT1 in Caco-2 cells. LACA, PAT1
67  2006 The extracellular pH dependency of transport activity by human oligopeptide transporter 1 (hPEPT1) expressed stably in Chinese hamster ovary (CHO) cells: a reason for the bell-shaped activity versus pH. CHO, hPepT1
68  2006 The functional role of arginine 901 at the C-terminus of the human anion transporter band 3 protein. DIDS, DNDS, HPG
69  2005 Binding residues and catalytic domain of soluble Saccharomyces cerevisiae processing alpha-glucosidase I. ER, NBS, TNM
70  2005 Effect of chemical modification of histidines on the copper-induced oligomerization of jack bean urease (EC JBU
71  2005 Mechanism of horseradish peroxidase-catalyzed heme oxidation and polymerization (beta-hematin formation). HRP
72  2004 Biochemical characterization of Plasmodium falciparum hypoxanthine-guanine-xanthine phosphorybosyltransferase: role of histidine residue in substrate selectivity. HGPRT, Pf
73  2004 Essential role of histidine 20 in the catalytic mechanism of Escherichia coli peptidyl-tRNA hydrolase. Pth
74  2004 Evidence for the presence of a critical histidine residue at the active site in glyceraldehyde-3-phosphate dehydrogenase of Ehrlich ascites carcinoma cells. EAC, GA3PD
75  2004 Functional reconstitution into proteoliposomes and partial purification of a rat liver ER transport system for a water-soluble analogue of mannosylphosphoryldolichol. DIDS, ER, Man-P-Dol
76  2004 Inhibition studies on Rhodospirillum rubrum H(+)-pyrophosphatase expressed in Escherichia coli. BPB, DCCD, FITC
77  2004 Molecular basis for modulation of recombinant alpha1beta2gamma2 GABAA receptors by protons. HEK
78  2004 Temperature effects on xylem sap osmolarity in walnut trees: evidence for a vitalistic model of winter embolism repair. ---
79  2003 Binding of the human "electron transferring flavoprotein" (ETF) to the medium chain acyl-CoA dehydrogenase (MCAD) involves an arginine and histidine residue. ETF, MCAD
80  2003 Chemical modification of contractile 3-nm-diameter filaments in Vorticella spasmoneme by diethyl-pyrocarbonate and its reversible renaturation by hydroxylamine. ---
81  2003 Chemical modifications of alpha1,6-fucosyltransferase define amino acid residues of catalytic importance. alpha6FucT, NEM, PCMB, PLG, PLP
82  2003 Critical role of histidine residues in cyclohexanone monooxygenase expression, cofactor binding and catalysis. CMO, FAD
83  2003 Evidence for essential histidine and dicarboxylic amino-acid residues in the active site of UDP-glucose : solasodine glucosyltransferase from eggplant leaves. ---
84  2003 His1205 and His1223 are essential for the activity of the mitogenic Pasteurella multocida toxin. PMT
85  2003 Histidine-834 of human erythrocyte band 3 has an essential role in the conformational changes that occur during the band 3-mediated anion exchange. DNDS
86  2003 Identification of the essential histidine residue for high-affinity binding of AlbA protein to albicidin antibiotics. ---
87  2003 Impact of purified water quality on molecular biology experiments. DHPLC, SNP
88  2003 Membrane fusion induced by vesicular stomatitis virus depends on histidine protonation. VSV
89  2003 Rat kidney acylase I: further characterisation and mutation studies on the involvement of Glu 147 in the catalytic process. EDTA, FA-Met
90  2003 The structural and functional studies of His119 and His12 in RNase A via chemical modification. ASA, RNase A
91  2003 Transcellular transport of a highly polar 3+ net charge opioid tetrapeptide. ---
92  2002 Biochemical characterization of the Pseudomonas putida 3-hydroxyacyl ACP:CoA transacylase, which diverts intermediates of fatty acid de novo biosynthesis. max, PMSF
93  2002 Chemical modification of catalytic site of lipase from wheat germ: altered structure-activity profile. EDC, PMSF
94  2002 Diethylpyrocarbonate inhibition of vacuolar H+-pyrophosphatase possibly involves a histidine residue. ---
95  2002 Differential interactions of estrogens and antiestrogens at the 17 beta-hydroxyl or counterpart hydroxyl with histidine 524 of the human estrogen receptor alpha. ERalpha, RAL
96  2002 Insights into the interaction of human liver arginase with tightly and weakly bound manganese ions by chemical modification and site-directed mutagenesis studies. ---
97  2002 Mapping Cu(II) binding sites in prion proteins by diethyl pyrocarbonate modification and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometric footprinting. MALDI-TOF, PrP
98  2002 N-terminal sequence and distal histidine residues are responsible for pH-regulated cytoplasmic membrane binding of human AMP deaminase isoform E. AMPD3
99  2002 p-Aminohippurate transport at the apical membrane in the OK kidney epithelial cell line. PAH
100  2002 Probing the role of active site histidine residues in the catalytic activity of lacrimal gland peroxidase. LGP