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Abbreviation : WT
Long Form : wild-type enzyme
No. Year Title Co-occurring Abbreviation
2020 Parallel Strategy Increases the Thermostability and Activity of Glutamate Decarboxylase. GABA, GAD, PLP
2019 Attenuated substrate inhibition of a haloketone reductase via structure-guided loop engineering. ---
2019 Characterization of recombinant fructose-1,6-bisphosphatase gene mutations: evidence of inhibition/activation of FBPase protein by gene mutation. CE, F26BP, FBPase
2019 Improvement of the activity of l-asparaginase I improvement of the catalytic activity of l-asparaginase I from Bacillus megaterium H-1 by invitro directed evolution. epPCR
2018 Fundamental Insights into Proton-Coupled Electron Transfer in Soybean Lipoxygenase from Quantum Mechanical/Molecular Mechanical Free Energy Simulations. DM, PCET, QM/MM
2017 Improvement thermostability of Pseudoalteromonas carrageenovora arylsulfatase by rational design. ---
2015 The general base in the thymidylate synthase catalyzed proton abstraction. dTMP, dUMP, H-bond, KIEs, TSase
2014 Amino acid substitutions away from the RNase H catalytic site increase the thermal stability of Moloney murine leukemia virus reverse transcriptase through RNase H inactivation. MMLV RT, T/P
2012 Solvent accessibility in the distal heme pocket of the nitrosyl d(1)-heme complex of Pseudomonas stutzeri cd(1) nitrite reductase. EPR, NO, P. aeruginosa, P. stutzeri
10  2011 Structure, electronic properties and catalytic behaviour of an activity-enhancing CYP102A1 (P450(BM3)) variant. ---
11  2010 Insight into the mechanism of the stabilization of moloney murine leukaemia virus reverse transcriptase by eliminating RNase H activity. MMLV RT, T/P
12  2009 Role of the charged amino acid residues in the cytoplasmic loop between putative transmembrane segments 6 and 7 of Na+-ATPase of an alkaliphilic bacterium, Exiguobacterium aurantiacum. ---
13  2008 Effects of introducing negative charges into the molecular surface of thermolysin by site-directed mutagenesis on its activity and stability. ---
14  2004 Dioxygen reduction by bo-type quinol oxidase from Escherichia coli studied by submillisecond-resolved freeze-quench EPR spectroscopy. ---
15  2003 Luminescent and substrate binding activities of firefly luciferase N-terminal domain. CoA
16  2002 Role of Thr(66) in porcine NADH-cytochrome b5 reductase in catalysis and control of the rate-limiting step in electron transfer. ---
17  2000 Importance of the free sulfhydryl groups of lecithin-cholesterol acyltransferase for its sensitivity to oxidative inactivation. LCAT
18  1999 Evidence for a two-base mechanism involving tyrosine-265 from arginine-219 mutants of alanine racemase. ---
19  1998 Engineering of a stable mutant malic enzyme by introducing an extra ion-pair to the protein. ---
20  1997 Chemical mechanism of the fructose-6-phosphate,2-kinase reaction from the pH dependence of kinetic parameters of site-directed mutants of active site basic residues. mantATP
21  1994 Effect of adding phosphorylation sites for cAMP-dependent protein kinase to rat testis 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase. ---
22  1994 Reaction energetics of a mutant 3-oxo-delta 5-steroid isomerase with an altered active site base (D38E). TIM
23  1992 Contribution to catalysis and stability of the five cysteines in Escherichia coli aspartate aminotransferase. Preparation and properties of a cysteine-free enzyme. AATase
24  1990 Kinetic investigation of the functional role of phenylalanine-31 of recombinant human dihydrofolate reductase. Phe31, rHDHFR
25  1987 Tyrosine 70 increases the coenzyme affinity of aspartate aminotransferase. A site-directed mutagenesis study. PLP