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Abbreviation: DIM
Appearance Frequency: 1321 time(s)
Long forms: 141

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Long Form No. Long Form Research Area Co-occurring Abbreviation PubMed/MEDLINE Info. (Year, Title)
days in milk
(663 times)
Veterinary Medicine
(582 times)
BCS (48 times)
DMI (47 times)
AI (39 times)
1997 Response of lactating dairy cows to various densities of sorghum grain.
3,3'-diindolylmethane
(379 times)
Neoplasms
(98 times)
I3C (98 times)
AhR (32 times)
ER (26 times)
1992 Acid reaction products of indole-3-carbinol and their effects on cytochrome P450 and phase II enzymes in rat and monkey hepatocytes.
daily intake of metals
(27 times)
Environmental Health
(20 times)
HRI (25 times)
Cd (6 times)
Cr (6 times)
2010 Soil and vegetables enrichment with heavy metals from geological sources in Gilgit, northern Pakistan.
dimycocerosates
(15 times)
Microbiology
(5 times)
Mtb (5 times)
PGL (3 times)
TB (2 times)
1974 Lipids of putative relevance to virulence in Mycobacterium tuberculosis: phthiocerol dimycocerosate and the attenuation indicator lipid.
diatomics-in-molecule
(10 times)
Chemistry
(10 times)
DMC (2 times)
DIIS (1 time)
HY (1 time)
2001 Molecular similarity for small species: refining the isoelectronic index.
dimer, 3,3'-diindolylmethane
(10 times)
Pharmacology
(3 times)
I3C (10 times)
AhR (1 time)
AR (1 time)
1991 Structure elucidation of acid reaction products of indole-3-carbinol: detection in vivo and enzyme induction in vitro.
dimeric product 3,3'-diindolylmethane
(10 times)
Antineoplastic Agents
(2 times)
I3C (9 times)
NF-kappaB (2 times)
AR (1 time)
2003 Gene expression profiles of I3C- and DIM-treated PC3 human prostate cancer cells determined by cDNA microarray analysis.
detergent-insoluble membrane
(8 times)
Biochemistry
(3 times)
APP (2 times)
CTFs (1 time)
DCC (1 time)
2000 The amino-terminal nine amino acid sequence of poliovirus capsid VP4 protein is sufficient to confer N-myristoylation and targeting to detergent-insoluble membranes.
dimethoate
(8 times)
Environmental Health
(3 times)
DIA (2 times)
DMSO (2 times)
ACE (1 time)
1986 In vitro induction of sister chromatid exchanges and chromosomal aberrations in peripheral lymphocytes of the oyster toadfish and American eel.
10  desipramine
(7 times)
Pharmacology
(4 times)
CORT (3 times)
NGF (2 times)
7-EC (1 time)
1999 Eight inhibitory monoclonal antibodies define the role of individual P-450s in human liver microsomal diazepam, 7-ethoxycoumarin, and imipramine metabolism.
11  1,4-dideoxy-1,4-imino-d-mannitol
(6 times)
Biochemistry
(5 times)
Endo H (1 time)
SW (1 time)
1985 1,4-Dideoxy-1,4-imino-D-mannitol inhibits glycoprotein processing and mannosidase.
12  driving-impairing medicines
(6 times)
Pharmacology
(2 times)
DRUID (2 times)
2017 Use of driving-impairing medicines by a Spanish population: a population-based registry study.
13  diminazene aceturate
(5 times)
Chemistry
(1 time)
ISMM (3 times)
b.wt (1 time)
CAC (1 time)
2004 Protective efficacy of isometamidium chloride and diminazene aceturate against natural Trypanosoma brucei, Trypanosoma congolense and Trypanosoma vivax infections in cattle under a suppressed tsetse population in Uganda.
14  detergent-insoluble microdomains
(4 times)
Biochemistry
(4 times)
Stx (2 times)
C/M (1 time)
CT (1 time)
1999 Activation of Src family kinase yes induced by Shiga toxin binding to globotriaosyl ceramide (Gb3/CD77) in low density, detergent-insoluble microdomains.
15  di(1H-indol-3-yl)methane
(4 times)
Biology
(2 times)
DIMs (1 time)
I3C (1 time)
QR (1 time)
2003 Effect of chemopreventive compounds from Brassica vegetables on NAD(P)H:quinone reductase and induction of DNA strand breaks in murine hepa1c1c7 cells.
16  digital integration method
(4 times)
Ophthalmology
(2 times)
AMD (2 times)
ARM (1 time)
CNV (1 time)
2006 Geometrical confinements and depletion interactions.
17  dimenhydrinate
(4 times)
Molecular Biology
(2 times)
CIN (3 times)
CC (1 time)
CPP (1 time)
2010 Assessment of the rewarding effects of dimenhydrinate using the conditioned place preference paradigm in mice.
18  distance between implant shoulder and mucosal margin
(4 times)
Dentistry
(3 times)
AL (2 times)
mBI (2 times)
mPLI (2 times)
2003 Effect of intracrevicular restoration margins on peri-implant health: clinical, biochemical, and microbiologic findings around esthetic implants up to 9 years.
19  domain information model
(4 times)
Medical Informatics
(3 times)
RIM (2 times)
AWG-OHI (1 time)
BP (1 time)
2000 PACS/information systems interoperability using Enterprise Communication Framework.
20  detergent-insoluble material
(3 times)
Cell Biology
(2 times)
DISAM (1 time)
GPA (1 time)
GPC (1 time)
1984 Glycosphingolipids in detergent-insoluble substrate attachment matrix (DISAM) prepared from substrate attachment material (SAM). Their possible role in regulating cell adhesion.
21  dimer
(3 times)
Chemistry
(3 times)
AA (1 time)
DE (1 time)
DMAc (1 time)
2003 Effect of the pyrrole polymerization mechanism on the antioxidative activity of nonenzymatic browning reactions.
22  data inconsistency metric
(2 times)
Diagnostic Imaging
(1 time)
CT (1 time)
2018 An Empirical Data Inconsistency Metric (DIM) Driven CT Image Reconstruction Method.
23  Days In Milking
(2 times)
Biosensing Techniques
(2 times)
EC (2 times)
LS (2 times)
SCC (2 times)
2015 Evaluation of the Fourier Frequency Spectrum Peaks of Milk Electrical Conductivity Signals as Indexes to Monitor the Dairy Goats' Health Status by On-Line Sensors.
24  Department of Internal Medicine
(2 times)
Health Services
(1 time)
HTCC (1 time)
IA (1 time)
KSW (1 time)
2004 Hospital readmissions--are they predictable and avoidable?
25  Department of Medical Informatics
(2 times)
Pediatrics
(1 time)
IMD (1 time)
SHA (1 time)
1996 Extraction and anonymity protocol of medical file.
26  dermatophyte identification medium
(2 times)
Microbiology
(2 times)
DTM (1 time)
1997 A new medium for the presumptive identification of dermatophytes.
27  difference in mean
(2 times)
Neurology
(1 time)
CI (2 times)
OKS (2 times)
FJS (1 time)
2019 A randomized trial to assess the immediate impact of acupuncture on quantitative sensory testing, pain, and functional status.
28  difference in means
(2 times)
Cardiology
(1 time)
APC (2 times)
CI (1 time)
CT (1 time)
2010 APCAP--activated protein C in acute pancreatitis: a double-blind randomized human pilot trial.
29  differential image motion
(2 times)
Optics and Photonics
(2 times)
DCIM (1 time)
2007 Experimental validation of the differential image motion lidar concept.
30  diffuse-interface model
(2 times)
Physical Phenomena
(2 times)
--- 2020 Dependence of the surface tension and contact angle on the temperature, as described by the diffuse-interface model.
31  digestibility index marker
(2 times)
Veterinary Medicine
(2 times)
AID (2 times)
ATTD (1 time)
BW (1 time)
2018 Digestibility index marker type, but not inclusion level affects apparent digestibility of energy and nitrogen and marker recovery in growing pigs regardless of added oat bran.
32  digital image microscopy
(2 times)
Cell Biology
(2 times)
ISIT (1 time)
MgTX (1 time)
1989 A digital image microscopy system for rare-event detection using fluorescent probes.
33  dimaprit
(2 times)
Neurology
(1 time)
2-TEA (1 time)
4-MeHA (1 time)
cGMP (1 time)
1986 Histaminergic stimulation of prolactin secretion mediated via H1- or H2-receptors: dependence on routes of administration.
34  dimerization
(2 times)
Medicine
(1 time)
HMG (1 time)
HMG-box (1 time)
2015 SOXE transcription factors form selective dimers on non-compact DNA motifs through multifaceted interactions between dimerization and high-mobility group domains.
35  dimethindene
(2 times)
Chemistry Techniques, Analytical
(1 time)
PHM (2 times)
CE-beta-CD (1 time)
cITP (1 time)
2007 Chiral separation of alkylamine antihistamines in pharmaceuticals by capillary isotachophoresis with charged cyclodextrin.
36  dimycocerosyl phthiocerol
(2 times)
Tropical Medicine
(1 time)
IFN-gamma (1 time)
LC (1 time)
MS (1 time)
1990 Effect of Mycobacterium leprae's phenolic glycolipid-I on interferon-gamma augmentation of monocyte oxidative responses.
37  Drug-Involved Mortality
(2 times)
Epidemiology
(1 time)
ED (1 time)
ICD-10 (1 time)
NDI (1 time)
2019 A comparison of opioid-involved fatalities captured in the National Poison Data System to data derived from US death certificate literal text.
38  dynamic impact modulus
(2 times)
Physiology
(1 time)
AS (1 time)
DZ (1 time)
PG (1 time)
2003 Influence of stress magnitude on water loss and chondrocyte viability in impacted articular cartilage.
39  ((R,S)-N,N-dimethyl-3(1(2-pyridyl)ethyl)indene-2-ethylamine)
(1 time)
Chemistry Techniques, Analytical
(1 time)
beta-CD (1 time)
CE (1 time)
ESI-MS (1 time)
2000 Mechanistic study of opposite migration order of dimethindene enantiomers in capillary electrophoresis in the presence of native beta-cyclodextrin and heptakis(2,3,6-tri-O-methyl)-beta-cyclodextrin.
40  1-Deoxymannojirimycin
(1 time)
Virology
(1 time)
RSV (1 time)
SW (1 time)
1985 The mannosidase inhibitors 1-deoxymannojirimycin and swainsonine have no effect on the biosynthesis and infectivity of Rous sarcoma virus.
41  2,3-dimethoxy-1,4-naphthoquinone
(1 time)
Pharmacology
(1 time)
DTD (1 time)
MEN (1 time)
NQ (1 time)
1999 Quinone toxicity in DT-diaphorase-efficient and -deficient colon carcinoma cell lines.
42  4-((3,4-dihydro-6,7-dimethoxyisoquinolin-1-yl)methyl)-6-methyl-2H-chromen-2-one
(1 time)
Chemistry Techniques, Analytical
(1 time)
DMB (1 time)
TMC (1 time)
2017 Study of Photophysical Properties on Newly Synthesized Coumarin Derivatives.
43  9,10-dimethylanthracene
(1 time)
Chemistry
(1 time)
ah (1 time)
AMI (1 time)
ANT (1 time)
2001 Rapid simultaneous determination of four anthracene derivatives using a single non-linear variable-angle synchronous fluorescence spectrum.
44  daily index of mucositis
(1 time)
Neoplasms
(1 time)
--- 1996 Quantitative scale of oral mucositis associated with autologous bone marrow transplantation.
45  daily intake mean
(1 time)
Environmental Health
(1 time)
ADI (1 time)
DIP (1 time)
2016 Spatial distribution and health risk assessment for groundwater contamination from intensive pesticide use in arid areas.
46  daily intake of heavy metals
(1 time)
Environmental Health
(1 time)
BCF (1 time)
DWI (1 time)
GWI (1 time)
2018 Spatial distribution of heavy metals in crops in a wastewater irrigated zone and health risk assessment.
47  days after lambing
(1 time)
BCS (1 time)
BW (1 time)
NEFA (1 time)
2020 Residual Feed Intake in Dairy Ewes: An Evidence of Intraflock Variability.
48  days in milk classes
(1 time)
Veterinary Medicine
(1 time)
BT (1 time)
BTCORR (1 time)
THI (1 time)
2010 Computing options for multiple-trait test-day random regression models while accounting for heat tolerance.
49  days in milk dry matter intake
(1 time)
Food
(1 time)
BW (1 time)
LDL (1 time)
2019 Increasing Selenium and Vitamin E in Dairy Cow Milk Improves the Quality of the Milk as Food for Children.
50  days of milk production
(1 time)
Animal Diseases
(1 time)
RT (1 time)
2018 Evaluation of rumination time, subsequent yield, and milk trait changes dependent on the period of lactation and reproductive status of dairy cows.
51  death by 1,1-bis(3'-indolyl)methane
(1 time)
Neoplasms
(1 time)
5,5'-diBrDIM (1 time)
2005 Inhibition of breast cancer cell growth and induction of cell death by 1,1-bis(3'-indolyl)methane (DIM) and 5,5'-dibromoDIM.
52  Deep InfoMax
(1 time)
Medical Informatics
(1 time)
AUROC (1 time)
BERT (1 time)
EHR (1 time)
2021 Disease Concept-Embedding Based on the Self-Supervised Method for Medical Information Extraction from Electronic Health Records and Disease Retrieval: Algorithm Development and Validation Study.
53  deep inspiration maneuver
(1 time)
Cardiology
(1 time)
PP (1 time)
SB (1 time)
VE (1 time)
2011 Hemodynamic changes during a deep inspiration maneuver predict fluid responsiveness in spontaneously breathing patients.
54  defocus-induced myopia
(1 time)
Optometry
(1 time)
CS (1 time)
DIH (1 time)
FDM (1 time)
2001 Comparison of refractive state and circumferential morphology of retina, choroid, and sclera in chick models of experimentally induced ametropia.
55  deicing materials
(1 time)
Public Health
(1 time)
--- 2015 [Methodical approaches to the substantiation of hygienic requirements for the application of deicing materials].
56  demonstrate that, mechanistically, T
(1 time)
Science
(1 time)
--- 2012 Division-linked generation of death-intermediates regulates the numerical stability of memory CD8 T cells.
57  density of virus infected midges
(1 time)
Virology
(1 time)
MLE (1 time)
SBV (1 time)
2019 Post-Epidemic Distribution of Schmallenberg Virus in Culicoides Arbovirus Vectors in Poland.
58  Dental Information Model
(1 time)
Dentistry
(1 time)
caDSR (1 time)
CDE (1 time)
PBRN (1 time)
2013 Using electronic dental record data for research: a data-mapping study.
59  dentin-derived inorganic mineral
(1 time)
Stem Cells
(1 time)
ALP (1 time)
BMMSCs (1 time)
2020 Dentin-Derived Inorganic Minerals Promote the Osteogenesis of Bone Marrow-Derived Mesenchymal Stem Cells: Potential Applications for Bone Regeneration.
60  depth of myometrial invasion
(1 time)
Medicine
(1 time)
CE (1 time)
FC (1 time)
LVI (1 time)
2017 Myometrium Invasion, Tumour Size and Lymphovascular Invasion as a Prognostic Factor in Dissemination of Pelvic Lymphatics at Endometrial Carcinoma.
61  derivatives, structurally related to 3,3'-methanediyl-bis-indole
(1 time)
Molecular Biology
(1 time)
EGFR (1 time)
2020 Theoretical evaluation of EGFR kinase inhibition and toxicity of di-indol-3-yl disulphides with anti-cancer potency.
62  design-types, hearing aids with inbuilt masker
(1 time)
Audiology
(1 time)
--- 2021 The Efficacy of Digital Hearing Aids in the Management of Tinnitus in Individuals with Sensorineural Hearing Loss.
63  desmethylimipramine
(1 time)
Pharmacology
(1 time)
CMC (1 time)
DDZ (1 time)
IM (1 time)
1988 [Drug interaction between imipramine hydrochloride and benzodiazepines when administered orally for 15 days].
64  details in motion
(1 time)
Image Processing, Computer-Assisted
(1 time)
MA (1 time)
MC (1 time)
ME (1 time)
2008 Deinterlacing using variational methods.
65  detect, identify, monitor
(1 time)
Public Health
(1 time)
MSc (1 time)
2013 The Southern African Centre for infectious disease surveillance: a one health consortium.
66  detectable I3C-derived product was 3,3'-diindolylmethane
(1 time)
Epidemiology
(1 time)
I3C (1 time)
2006 Single-dose and multiple-dose administration of indole-3-carbinol to women: pharmacokinetics based on 3,3'-diindolylmethane.
67  detergent-insoluble membrane microdomains
(1 time)
Biochemistry
(1 time)
ERK (1 time)
2007 A cholesterol-independent membrane microdomain serves as a functional counter-receptor for E-selectin at the Colo201 cell surface and initiates signalling on E-selectin binding.
68  dexamethasone, insulin, and isobutylmethylxanthine
(1 time)
Biotechnology
(1 time)
ASCs (1 time)
DH IH (1 time)
2013 The combination of DHEA, histamine, and insulin increases adipogenic differentiation and enhances tissue transplantation outcome in mice.
69  dexamethasone, insulin, methylisobutylxanthine
(1 time)
Biophysics
(1 time)
hMSCs (1 time)
2010 Development of a rapid culture method to induce adipocyte differentiation of human bone marrow-derived mesenchymal stem cells.
70  di-imidazole lexitropsin
(1 time)
Biotechnology
(1 time)
Im (1 time)
Py (1 time)
1997 Defining GC-specificity in the minor groove: side-by-side binding of the di-imidazole lexitropsin to C-A-T-G-G-C-C-A-T-G.
71  Diabetes Infection Measure
(1 time)
Medical Informatics
(1 time)
DDA (1 time)
DDC (1 time)
DME (1 time)
2020 Internet of things-inspired healthcare system for urine-based diabetes prediction.
72  Diabetes Integrated Management
(1 time)
Endocrinology
(1 time)
DSC (1 time)
T2D (1 time)
2018 Outcomes of integrated management versus specialized care for patients with type 2 diabetes: An observational study.
73  diagnosis time
(1 time)
Veterinary Medicine
(1 time)
BCS (1 time)
BHB (1 time)
JD (1 time)
2007 The associations between culling due to clinical Johne's disease or the detection of Mycobacterium avium subsp. paratuberculosis fecal shedding and the diagnosis of clinical or subclinical diseases in two dairy herds in Minnesota, USA.
74  diagnostic imaging
(1 time)
Chiropractic
(1 time)
IRT (1 time)
2020 The transition to digital presentation of the diagnostic imaging domain of the Part IV examination of the National Board of Chiropractic Examiners.
75  Diagnostic Instrument Manipulator
(1 time)
Biophysics
(1 time)
NIF (1 time)
PPPL (1 time)
2018 Absolute calibration of a time-resolved high resolution x-ray spectrometer for the National Ignition Facility (invited).
76  diagonal incisor malocclusion
(1 time)
Veterinary Medicine
(1 time)
EMC (1 time)
2007 A retrospective evaluation of 204 diagonal incisor malocclusion corrections in the horse.
77  diamorphine
(1 time)
Toxicology
(1 time)
6-MAM (1 time)
LC-MS-MS (1 time)
SPE (1 time)
2010 Method for the quantification of diamorphine and its metabolites in pediatric plasma samples by liquid chromatography-tandem mass spectrometry.
78  diastolic inward motion
(1 time)
Diagnostic Imaging
(1 time)
Cx (1 time)
LAD (1 time)
LV (1 time)
1981 Echocardiographic analysis of systolic and diastolic left ventricular wall motion during transient myocardial ischemia.
79  DICOM Image Management
(1 time)
Medical Informatics
(1 time)
DICOM (1 time)
2006 A service based approach for medical image distribution in healthcare Intranets.
80  diet-induced animal model of metabolic syndrome
(1 time)
Nutritional Physiological Phenomena
(1 time)
SGP (1 time)
2013 Role of standardized grape polyphenol preparation as a novel treatment to improve synaptic plasticity through attenuation of features of metabolic syndrome in a mouse model.
81  dietary indoles, 3,3'-diindolylmethane
(1 time)
Immune System Diseases
(1 time)
AhR (1 time)
I3C (1 time)
Th17 (1 time)
2019 Indole Treatment Alleviates Intestinal Tissue Damage Induced by Chicken Coccidiosis Through Activation of the Aryl Hydrocarbon Receptor.
82  dietary modification
(1 time)
Complementary Therapies
(1 time)
DBP (1 time)
PE (1 time)
PR (1 time)
2014 Yoga for hypertension: a systematic review of randomized clinical trials.
83  dietetics and integrative medicine
(1 time)
Nutritional Sciences
(1 time)
--- 2015 The Next Generation of Dietitians: Implementing Dietetics Education and Practice in Integrative Medicine.
84  diethyl isopropylidene malonate
(1 time)
Chemistry
(1 time)
CO (1 time)
2010 Synthesis, characterization, and catalytic properties of new electrophilic iridium(III) complexes containing the (R)-(+)-2,2'-bis(diphenylphosphino)-1,1'-binaphthyl ligand.
85  different days of milk
(1 time)
Veterinary Medicine
(1 time)
ACC (1 time)
FA (1 time)
FAS (1 time)
2009 Sheep and goats differences in CLA and fatty acids milk fat content in relation with mRNA stearoyl-CoA desaturase and lipogenic genes expression in their mammary gland.
86  different implant manufacturer
(1 time)
Orthopedics
(1 time)
rTKA (1 time)
TKA (1 time)
2021 Patellar Tracking in Revision Total Knee Arthroplasty: Does Retaining a Patella From a Different Implant System Matter?
87  differential importance
(1 time)
Statistics as Topic
(1 time)
PSA (1 time)
2008 Epistemic uncertainty in the ranking and categorization of probabilistic safety assessment model elements: issues and findings.
88  differential interference microscope
(1 time)
Pharmacy
(1 time)
alpha-CD (1 time)
cryo-SEM (1 time)
HPLC (1 time)
2016 [Formation mechanism of Pickering emulsions induced by self-assembly of medium chain triglycerides and alpha-cyclodextrin].
89  differentiating mixture
(1 time)
Endocrinology
(1 time)
HFD (1 time)
MetS (1 time)
RD (1 time)
2012 Testosterone treatment improves metabolic syndrome-induced adipose tissue derangements.
90  differentiation-inducing medium
(1 time)
Clinical Laboratory Techniques
(1 time)
hUMSC (1 time)
LC-CM (1 time)
2017 Differentiation of human umbilical cord mesenchymal stem cells into steroidogenic cells in vitro.
91  diffuse iris melanomas
(1 time)
PT (1 time)
2019 Diffuse Iris Melanoma: Conservative Treatment with Proton Beam Therapy after Limbal Stem Cell Preservation or Enucleation?
92  diffuse iris ring melanoma
(1 time)
Medical Oncology
(1 time)
GEP (1 time)
2021 Locally Invasive Diffuse Iris Ring Melanoma Presenting as Unilateral Severe Glaucoma: Case Report and Review of Molecular Genetics.
93  digital image manipulation
(1 time)
Sports Medicine
(1 time)
MTMM (1 time)
2006 Multitrait-multimethod investigation of a novel body image measurement technique.
94  digital implicit model
(1 time)
Ophthalmology
(1 time)
GGSM (1 time)
GSM (1 time)
SSE (1 time)
2020 Echo decomposition of full-waveform LiDAR based on a digital implicit model and a particle swarm optimization.
95  Digital Intensity Model
(1 time)
Biological Science Disciplines
(1 time)
GIS (1 time)
RS (1 time)
2014 Automatic recognition of seismic intensity based on RS and GIS: a case study in Wenchuan Ms8.0 earthquake of China.
96  diiodomethane
(1 time)
Molecular Biology
(1 time)
HSA (1 time)
IgG (1 time)
PEI (1 time)
2006 Surface energy components of a dye-ligand immobilized pHEMA membranes: effects of their molecular attracting forces for non-covalent interactions with IgG and HSA in aqueous media.
97  DIM in oil solution
(1 time)
Preventive Health Services
(1 time)
AUC (1 time)
2013 Comparative preclinical pharmacokinetics study of 3,3'-diindolylmethane formulations: is personalized treatment and targeted chemoprevention in the horizon?
98  dimcthachlon
(1 time)
Environmental Health
(1 time)
PAL (1 time)
PPO (1 time)
S. sclerotiorum (1 time)
2020 Enhancement and improvement of selenium in soil to the resistance of rape stem against Sclerotinia sclerotiorum and the inhibition of dissolved organic matter derived from rape straw on mycelium.
99  dimerized
(1 time)
Medicine
(1 time)
--- 2018 Correlation-Driven Dimerization and Topological Gap Opening in Isotropically Strained Graphene.
100  Dimetapp
(1 time)
Emergency Medicine
(1 time)
BPM (1 time)
PEH (1 time)
PL (1 time)
1983 Acute otitis media in children: are decongestants or antihistamines necessary?